Calcium-free phosphate-buffered saline (PBS) is a widely used laboratory buffer designed for cell culture, molecular biology, and biomedical research applications. Researchers in Ames use calcium-free PBS for washing cells, preparing samples, diluting reagents, and performing procedures where calcium ions could interfere with cell dissociation or experimental results. Many formulations are also free of magnesium, making them suitable for use with enzymes such as trypsin during cell harvesting. Available in sterile and non-sterile forms, calcium-free PBS is offered in various concentrations and container sizes to meet different laboratory requirements. When selecting a PBS solution, consider sterility, pH, osmolarity, formulation, and compatibility with your specific protocol to ensure consistent performance and reliable experimental outcomes.